Studying the scientific basis of specific correlation between the meridian and Zang Fu organ by using gene differential expression in the rat. 21 SD rats were randomly and evenly divided into control group, sham-myocardial ischemia (MI) group, electroacupuncture-heart-meridian (HT) group, EA-lung-meridian group (LU), and EA-small-intestine-meridian group (SI) group. EA was applied to
Shenmen (HT7) Shu-Stream, Yuan-Primary Point and 5,
Yanglao (SI6) Xi-Cleft Point and 7,
Taiyuan (LU9)Shu-Stream Point, Yuan-Source Point and 7 for 20 min, once daily and continuously for 3 days. Myocardial ischemia model was duplicated by occlusion of the anterior descending branch of the left coronary artery, and myocardial tissue of the left ventricle was taken 3 days after EA treatment for analyzing the differentially expressed genes in different group with rat whole gene U 230 sequence chips.
Results: Comparing with model group, among the differentially expressed genes being twofold and above, the up-regulated and the down-regulated were 20 and 70 in EA-HT group, 18 and 26 in EA-SI group, and 14 and 20 in EA-LU group separately. Few same genes with the same differential expression were found among the three EA groups.
Conclusion: There is exactly a molecular basis in the correlation between heart meridian and the heart, and EA of heart meridian, small intestine meridian and lung meridian have their own special signaling pathway.
Key Words: differentially expressed genes, relatively specific connection, heart meridian and heart
Writer: Yiping Zhou, Keming Wang, Ling Hu, Zijian Wu, Meiqi Zhou, Yuelan Wang, Huangtian Yang, Fabao Zhang, Lu Sun
Center for Researching Correlation Between Meridians and Zangfu-organs, Anhui College of Chinese medicine, Hefei 230038, China;
Institute of Health Sciences, Shanghai Academy of Life Science, Chinese Academy of Science, Shanghai 200025;
Shanghai Gene Tech Biotechnology Company Limited, Shanghai 200223
Comments: